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The Body Wall and Musculature of the Marine Triclad Palombiella stephensoni (Palombi, 1938): Part One: General Tissue Structure as seen with the Light Microscope


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It was found that bufferred 4% formaldehyde and picroformalin were unsuitable fixatives for preservation of the epidermis. In worms fixed in these substances the epidermis was frequently broken when seen in sections, and the cells were shrunken and distorted. Cellular detail was very difficult to see because of this distortion. By far the best preservation of form was given by Lillie's AAF. When this fixative was used the epidermis was seen as a fairly uniform layer of cells. Cilia were well preserved. Carnoy's fluid also gave good preservation of the epidermis, but not as good as the AAF of Lillie.